Fig. 2

Effect of IAV H1N1 infection on miRNA expression in pBECs from non-asthmatics and asthmatics cultured at ALI. Cells were infected with IAV H1N1 (MOI 5) and the expression of candidate miRNAs were assessed at different time points (0, 6, 8 and 24 h). a miR-20a, (b) miR-132, and (c) miR-22, were expressed at similar levels in non-asthmatics and asthmatic cells, at baseline (0 h). H1N1 infection had no effect on the expression of (a) miR-20a, and (b) miR-132, whereas (c) miR-22 expression increased after infection in cells form non-asthmatics but not in asthmatics, *P ≤ 0.05, using the Kruskal Wallis multiple comparisons test, N = 5. The cycle threshold (Ct) value was normalized to that of the RNU44 (∆Ct). Data are presented relative to corresponding non-asthmatic levels at baseline