Fig. 1

Effect of IAV H1N1 infection on miRNA expression in pBECs from non-asthmatics and asthmatics cultured as monolayers. Cells were infected with IAV H1N1 (MOI 5) and the expression of candidate miRNAs were assessed at different time points (0, 1, 4, 6 and 24 h). a miR-20a, (b) miR-132, and (c) miR-22, were expressed at similar levels in non-asthmatics and asthmatic cells, at baseline (0 h) or after IAV H1N1 infection (1–24 h), using Kruskal Wallis multiple comparisons test, N = 5. The cycle threshold (Ct) value was normalized to that of the RNU44 (∆Ct). Data are presented relative to corresponding non-asthmatic levels at baseline