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Table 3 Results from meta-analysis, FEV1/FVC and AAT, adult cohorts

From: SERPINA1 methylation and lung function in tobacco-smoke exposed European children and adults: a meta-analysis of ALEC population-based cohorts

CpG Position Gene Group SAPALDIA Meta- Analysis of Lung Function Association in SAPALDIA, NFBC and ECRHS
Circulating AAT Cross-Sectional T1 Cross-Sectional T2 Repeat Cross-Sectional Change
Coef. P.valuea Coef. P.valuea Directionb Coef. P.valuea Directionb Coef. P.valuea Directionb Coef. P.valuea Directionb
cg10761141 94,844,776 3UTR −0.30 0.25 0.10 0.14 −++ −0.02 0.79 + − + −0.01 0.79 – − + 0.00 0.82 + − –
cg25042671 94,849,061 Body −0.59 0.04 −0.09 0.29 − + − − 0.12 0.22 – – – −0.12 0.03 + − – 0.00 0.62 + − +
cg20267408 94,849,703 5UTR −0.44 0.21 −0.05 0.57 – – – 0.05 0.59 −++ 0.01 0.88 + − + 0.00 0.78 −++
cg26938334 94,849,747 5UTR 1.16 0.42 0.10 0.24 − + − −0.10 0.47 −++ 0.01 0.84 ++− −0.01 0.33 – − +
cg10832639 94,850,965 5UTR −0.25 0.72 0.16 0.14 + − + 0.02 0.86 − + − 0.08 0.18 +++ 0.01 0.32 +++
cg02126235 94,855,034 5UTR −0.30 0.58 0.26 0.06 +++ 0.06 0.71 − + − 0.06 0.41 ++− 0.00 0.90 – − +
cg09968361 94,855,344 5UTR −0.33 0.44 0.11 0.23 +++ 0.00 0.99 ++− 0.05 0.25 ++− −0.01 0.37 – − +
cg13826459 94,855,505 TSS1500 −0.08 0.76 0.07 0.34 + − + 0.15 0.05 + − + 0.04 0.32 +++ 0.01 0.24 −++
cg25968219 94,855,590 TSS1500 −1.81 0.05 0.31 0.02 +++ −0.16 0.43 – − + − 0.01 0.90 −++ 0.00 0.59 + − +
cg01606800 94,855,990 TSS1500 −1.08 0.001 0.17 0.05 + − + 0.01 0.96 – − + 0.00 0.97 – − + 0.00 0.73 ++−
cg02181506 94,856,984 1stExon −0.78 0.08 −0.01 0.89 + − – 0.05 0.64 + − – −0.03 0.58 + − – 0.00 0.80 ++−
cg10070185 94,857,151 TSS200 −0.05 0.91 0.07 0.45 +++ 0.19 0.09 −++ −0.01 0.81 – − + 0.00 0.63 – − +
cg24621042 94,857,275 TSS1500 −0.68 0.04 0.18 0.02 +++ 0.16 0.08 +++ 0.07 0.09 +++ −0.01 0.09 + − –
cg00802237 94,858,066 TSS1500 −0.07 0.81 0.03 0.71 + − + −0.14 0.09 – – – 0.00 0.96 −++ 0.00 0.50 + − –
cg04179148 94,858,339 TSS1500 −0.70 0.06 0.20 0.03 +++ 0.04 0.73 ++− 0.12 0.02 +++ 0.00 0.57 – – –
  1. Cross-Sectional and repeat cross-sectional models adjusted for: study center, age, age2, education, height, height2, sex, sex*age, (sex*age) 2, sex*height, (sex*height)2, Bcell, CD4T, CD8T, Eos, Mono, NK. Repeat cross-sectional in addition ran with a random intercept on the subject. In predictive models, annual change in lung function was computed as the difference between T2 and T1 divided by time of follow-up: (T2-T1)/follow-up, models adjusted for covariates from T1
  2. Sample size for circulating AAT for SAPALDIA n = 561. Cross-sectional and prediction models (change) n = 1076, and n = 1122 for repeat cross-sectional models
  3. Results from Meta-Analysis of Lung Function Association of Methylation of CpGs in the SERPINA1* Gene with FEV1/FVC Level and Decline in Adult Ever-Smokers from SAPALDIA, ECRHS and NFBC and with Circulating AAT Concentrations in SAPALDIA
  4. From SERPINA gene cluster located on chromosome 14 between positions: 94′’641’781 and 95′’235’125 (human genome build 37/hg19)
  5. aNominal P-values, Bonferroni corrected significance level for 119 tests is P-value 4.6 × x10-4
  6. bDirection of effect for SAPALDIA, ECRHS and NFBC. A positive sign indicates that an increase in methylation is associated with higher level of lung function (cross-sectional models) and with an attenuation of lung function decline, respectively (change model)
  7. Statistically significant results are highlighted in bold