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Fig. 1 | Respiratory Research

Fig. 1

From: Asthmatic bronchial epithelial cells promote the establishment of a Hyaluronan-enriched, leukocyte-adhesive extracellular matrix by lung fibroblasts

Fig. 1

Expression of hyaluronan synthase (HAS) isoforms is increased in human lung fibroblasts (HLFs) alone (N = 10) compared to HLFs co-cultured with bronchial epithelial cells (BECs) obtained from healthy (N = 10) or asthmatic donors (N = 11). a HAS2 expression was significantly greater in HLFs co-cultured with asthmatic BECs compared to HLFs co-cultured with healthy BECs (P = 0.02). Expression of HAS2 was increased in both HLFs co-cultured with healthy BECs and asthmatic BECs compared to HLFs alone (P < 0.0001 for both comparisons). b Expression of HAS3 was significantly greater by HLFs co-cultured with asthmatic BECs compared to HLFs co-cultured with healthy BECs (P = 0.02). HAS3 expression was increased in HLFs co-cultured with asthmatic BECs (P = 0.005), but not in HLFs co-cultured with healthy BECs compared to HLFs alone. Expression of hyaluronan (HA) degradation enzymes c HYAL1, d HYAL2, and co-receptor e CD44 were not significantly different between HLFs co-cultured with healthy BECs and HLFs co-cultured with asthmatic BECs for each comparison. However, expression of HYAL1, HYAL2, and CD44 was found to be elevated in HLFs in both healthy and asthmatic co-cultures compared to HLFs alone (P < 0.001 for each comparison). Gene expression was normalized to GAPDH and is shown as normalized mean ± SD relative to HLF alone

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