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Fig. 3 | Respiratory Research

Fig. 3

From: Human alveolar epithelial cells type II are capable of TGFβ-dependent epithelial-mesenchymal-transition and collagen-synthesis

Fig. 3

TGFβ induces actin cytoskeleton remodeling in hAECII. hAECII (N = 5) were seeded on coverslips and cultivated for 48 h in medium a, 5 ng/ml TGFβ b, 10 μM SB431542 (c) or both (d). The actin cytoskeleton was visualized by Rhodamine-Phalloidin and nuclei by DAPI. A representative image from one experiment shows the actin cytoskeleton at a magnification of 200× with a scale bar of 100 μm. Quantification of the actin area per cell was done with ImageJ and depicted as scatter dot plot (E) with the mean value and SD of 5 biological replicates. For statistical analysis, p ≤ 0.05 (*), 0.01 (**) and 0.001 (***) were regarded as significant

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Respiratory Research

ISSN: 1465-993X

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