Fig. 1

Transcriptional analysis of hAECII upon TGFβ stimulation. hAECII different donors were stimulated each with 5 ng/ml TGβ1, 10 μM SB431542, 5 ng/ml TGFβ1 and 10 μM SB431542 or left untreated for 48 h (N = 4). Significantly regulated genes were computed by Repeated-Measures ANOVA (RM-ANOVA) with a Benjamini-Hochberg multiple testing correction cut-off of ≤0.05. Averaged gene expression data of results from RM-ANOVA is shown as a Heatmap with Hierarchical Clustering of samples and entities according to Pearson Centered algorithm with Ward’s linkage rule. A Fold-Change Filter was applied to further analyze only probes that were at least ≥2 fold up-regulated compared to medium control (a). Out of those genes, a list of targets was extracted that was exclusively regulated by TGFβ and designated TGFβ fingerprint (b). The TGFβ fingerprint genes were further investigated for mutual interactions by querying the String protein-protein interaction database with those genes that exhibited an annotated GeneSymbol. A global map of these genes was constructed based on interaction scores within Cytoscape (c). The presence of intrinsic modules within the interactions of the TGFβ fingerprint was computed by a spectral partition based cluster algorithm from the Reactome FIViz app and respective modules encoded by different colours. Further details of interactions within each module are shown in (d). Global enrichment of GSEA Hallmark gene sets (e) and Reactome pathways (f) from whole list of TGFβ fingerprint genes are displayed by their FDR q-value