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Table 1 Relative mRNA expression associated with endothelial cell damage

From: Changes in pulmonary endothelial cell properties during bleomycin-induced pulmonary fibrosis

 

Day 7

Day 21

Saline

Bleomycin

Saline

Bleomycin

vWF

1.19 ± 0.25

3.09 ± 0.15*

1.33 ± 0.22

2.76 ± 0.14*

MMP-12

1.39 ± 0.20

18.0 ± 0.37*

0.87 ± 0.37

70.3 ± 0.52*, **

PAI-1

1.63 ± 0.31

6.05 ± 0.28*

1.72 ± 0.14

4.58 ± 0.32*

  1. The table shows expression of endothelial damage markers, von Willebrand factor (vWF), matrix metalloproteinase (MMP)-12 and plasminogen activator inhibitor-1 (PAI-1) in lung endothelial cells isolated from saline-treated and bleomycin-treated mice. mRNA levels were determined by quantitative real time-PCR. Total RNA was isolated from magnetically sorted CD45−CD31+ mouse lung endothelial cells at 7 and 21 days after saline or bleomycin administration. PCR was performed using three or four independently prepared cDNA samples from endothelial cells. Results were normalized to expression levels in endothelial cells from untreated lungs at day 0 and are expressed as fold-changes. Data are means ± standard error, from three or four mice
  2. *p < 0.05, compared with saline-treated mice on the same day
  3. **p < 0.01, compared with bleomycin-treated mice on day 7
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Respiratory Research

ISSN: 1465-993X

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