Fig. 6From: Comparison of pro- and anti-inflammatory responses in paired human primary airway epithelial cells and alveolar macrophagesPAMP stimulation induces expression of negative regulators of TLR signaling in alveolar macrophages. mRNA expression of the negative regulators Tollip (a), A20 (b) and IRAK-M (c) in macrophages in the absence (−) and presence of LPS, Poly(I:C) (PIC) at 4, 24 and 48 h. N = 8 donor subjects. Median values are shown as horizontal lines. Densitometric analysis of Tollip (d) and A20 (e) western blots on lysates of macrophages in the absence (−) or presence of treatments with LPS, PIC at 4, 24 and 48 h. β-actin was included as a protein loading control to normalize Tollip or A20 expression. N = 3 donor subjects (additional blots in Additional file 1: Figure S2)Back to article page