Fig. 5From: Comparison of pro- and anti-inflammatory responses in paired human primary airway epithelial cells and alveolar macrophagesPAMP stimulation induces expression of negative regulators of TLR signaling in human tracheobronchial epithelial cells (TBEC). mRNA expression of negative regulators Tollip (a), A20 (b) and IRAK-M (c) in TBEC in the absence (−) and presence of LPS or Poly(I:C) (PIC) at 4, 24 and 48 h. N = 8 donor subjects. Median values are shown as horizontal lines. Densitometric analysis of Tollip (d) and A20 (e) western blots on lysates of TBEC in the absence (−) or presence of treatments with LPS, PIC at 4, 24 and 48 h. β-actin was included as a protein loading control to normalize Tollip or A20 expression. N = 3 donor subjects (additional blots in Additional file 1: Figure S2)Back to article page