Fig. 1

Hsa_circ_0046264 was down-regulated in lung cancer tissues. a Differentially expressed circRNAs in lung cancer tissues were presented in a volcano plot. The screening condition was fold change > 2 and P < 0.05. b The heat map reflected 50 significantly differentially expressed circRNAs in lung cancer tissues. Hsa_circ_0046264 was lowly expressed in cancer tissues. c The overall survival (OS) of lung cancer patients with high or low circ_0046264 level. A low level predicted a worse prognosis outcome. d The expression level of hsa_circ_0046264 decreased with the deterioration of lung cancer (from stage I to stage IV). ^*P < 0.05, ^***P < 0.001, compared with I (N = 5) group with lung cancer tissues in Stage I. e-f RNase R can digest liner RNA GAPDH but not circRNA hsa_circ_0046264 as the relative expression of GADPH in lung cells decreased after RNase R digestion while that of hsa_circ_0046264 had no significant difference. ^**P < 0.01, ^***P < 0.001, compared with MOCK group. g The relative expression of hsa_circ_0046264 was lower in lung cancer cells A549 and 59-D than that in normal lung cells. ^*P < 0.05 compared with HLF-a cells