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Fig. 5 | Respiratory Research

Fig. 5

From: Rapid clearance of heavy chain-modified hyaluronan during resolving acute lung injury

Fig. 5

Effect of LPS on genes implicated in HA synthesis and breakdown in whole lung and alveolar macrophage. a-c. Expression of HA synthases (HAS1–3, a), hyaluronidases (HYAL1–2, b), transmembrane protein 2 (TMEM2) and cell migration-inducing and HA-binding CEMIP (c) was assessed by qPCR in lungs of mice instilled intratracheally with vehicle (PBS) or LPS for the indicated duration. Each data point represents an individual mouse. d-e. Expression of genes implicated in HA breakdown HYAL1, HYAL2, TMEM2, and CD44 in resident and recruited mouse alveolar macrophages isolated from bronchoalveolar lavage of mice treated with LPS (20 μg intratracheal; 0, 3, 6, 9, and 12 dpi). Expression level was assessed by RNA-seq and shown as transcripts per million (TPM). Mean (n = 3 independently pooled samples per time point, 4–7 mice for each pool) and SD plotted. NA, not applicable; SD, standard deviation. Data analyzed with ANOVA and Tukey’s multiple comparisons; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < .0001

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