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Fig. 4 | Respiratory Research

Fig. 4

From: Rapid clearance of heavy chain-modified hyaluronan during resolving acute lung injury

Fig. 4

Roughness of peribronchial HA following LPS. a. Identification of HA staining in peri-broncho-vascular interstitial areas bordered by blood vessels (V) and bronchi (Br) in paraformaldehyde fixed, frozen sections of lungs instilled with LPS (4 dpi) or control (PBS) and immunostained with HA-binding protein (green), antibody against CD68 (macrophage marker, white), and the nuclear stain DAPI (blue). A negative control for staining, using secondary antibody only is shown in the bottom row. Surface plots of the maximum intensity of staining (Z-projection) spanning 9.4 × 9.4 μm areas were generated, shown to the right, measured in arbitrary units (AU); Scale bar 50 μm. b. Representative line profile of HA staining in the max intensity Z-projection (50 × 50 μm). c. The normalized surface roughness of HA staining intensity was determined for both PBS and LPS (4 dpi) treated lungs by dividing the surface area of the intensity plots by the average staining intensity (n = 3 mice per group); ANOVA with Tukey’s multiple comparisons; *P < 0.05)

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