Fig. 3

Effect of LPS on lung HA molecular weight distribution. a. Detection of HA by Stains-All staining of agarose gel-resolved glycosaminoglycans extracted from lung tissue of mice following intratracheal instillation of vehicle (PBS) control or LPS for the indicated time. Extracted samples were examined prior to (−) and following hyaluronidase treatment (+). Select-HA consisting of 2500, 1000, 500, and 250 kDa HA and Select-HA HiLadder consisting of 1510, 1090, 966, 572, and 495 kDa HA were used to determine HA molecular weight. b. Distribution of HA abundance (mean +/− SEM) by molecular weight size, determined by Select-HA: PBS (n = 3), LPS 1 dpi (n = 4), LPS 2 dpi (n = 3). c. Levels of HA ranging from 1000 to 2500 kDa (HMW) or 250–500 kDa levels were determined by integrating the area of HA abundance over the specified molecular weight ranges for each individual mice. Each data point represents an individual mouse lung. n = 3–4 mice per group. Data analyzed with ANOVA and Tukey’s multiple comparisons, *P < 0.05, **P < 0.01. HMW, high molecular weight; SEM, standard error of the mean; AU, arbitrary unit