Fig. 4
From: Hypoargininemia exacerbates airway hyperresponsiveness in a mouse model of asthma
Similar induction of inflammatory genes in asthmatic hypoargininemic and control mice. Data from F/A2wt/wt (white bars) and F/A2tg/tg (black bars) mice are shown. Mice were either mock-sensitized with PBS before the ovalbumin (OVA) challenge (PBS/OVA), or sensitized and challenged with OVA (OVA/OVA) as indicated below panels I and J. Panels a-d: mRNA abundance of the TH2-related genes Il4, Il13, Il5 and Ccl11, respectively. Panels e and f: mRNA abundance of anti-inflammatory gene Il10 and macrophage-chemotactic gene Ccl2. Panels g and h: mRNA abundance of the TH1-related genes Tnfa and Ifng. Panels i and j: mRNA abundance of marker genes for activation of bronchiolar epithelium Muc5ac and Clca3. The Y-axis shows the number of mRNA copies after normalization to 18S rRNA expression and multiplication by 10,000. Means ± SEM of 7–8 mice per group. Significance symbols: *: P < 0.05 PBS/OVA vs. OVA/OVA F/A2wt/wt; #: P < 0.05 PBS/OVA vs. OVA/OVA F/A2tg/tg; ‡: P < 0.05 OVA/OVA F/A2tg/tg vs. F/A2wt/wt