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Fig. 4 | Respiratory Research

Fig. 4

From: Human antigen R enhances the epithelial-mesenchymal transition via regulation of ZEB-1 in the human airway epithelium

Fig. 4

ZEB-1 was required for HuR-mediated EMT in BEAS-2B cells. (A)*P < 0.05 based on a Student’s t-test. (B) Western blot was used to detect ZEB-1 and HuR expression. BEAS-2B cells were treated with 3% CSE for 48 h in the presence or absence of HuR gene silencing. ZEB-1 expression and its correlation with HuR expression in the airway epithelium. Immunohistochemical assessment of ZEB-1 expression in non-smoking subjects (n = 18), smokers without COPD (n = 20), and smokers with COPD (n = 30). (C) Representative ZEB-1 immunostaining (brown staining) in the airways of a nonsmoker (a), a smoker without COPD (b), and a smoker with COPD (c). (D) Quantification of ZEB-1 protein levels in the airway epithelium using the integrated optical mean density. The expression of ZEB-1 was correlated with the HuR expression in the airway epithelium of nonsmokers (E), smokers without COPD (F), and smokers with COPD (G) by Spearman’s correlation test. Western blot was used to detect the expression of ZEB-1, E-cadherin and vimentin (H).The values are given as the mean ± s.e.m. **P < 0.01 compared with nonsmokers and ##P < 0.01 compared with smokers without COPD

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