Fig. 3

HuR was necessary for CSE-induced EMT in BEAS-2B cells. HuR mRNA (a) and protein (b) expression levels were detected using real-time PCR and Western blot analysis, respectively, with three HuR-siRNA sequences and a negative control siRNA at 48 h after transfection. Each value is presented as the mean ± SD from three independent experiments. **P < 0.01 compared with cells transfected with siNC. c, d and e) BEAS-2B cells were treated with 3% CSE for 48 h in the presence or absence of HuR gene silencing. c Western blot was used to detect the expression of E-cadherin, vimentin, and HuR. d Immunofluorescence analysis of the expression of E-cadherin and vimentin using an inversion fluorescence microscope. e Cell images were obtained using phase-contrast microscopy