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Fig. 2 | Respiratory Research

Fig. 2

From: Human antigen R enhances the epithelial-mesenchymal transition via regulation of ZEB-1 in the human airway epithelium

Fig. 2

CSE increased HuR expression and enhanced the cytoplasmatic translocation of HuR in BEAS-2B cells. a HuR expression was examined by Western blot with various concentrations of CSE for 48 h. b HuR expression was examined by Western blot with 3% CSE for different time periods. c Immunofluorescence analysis of the subcellular distribution of HuR in BEAS-2B cells treated with 3% CSE for different time periods. The arrows refer to the cytoplasmic distribution of HuR. Western blot of HuR expression levels in the cytoplasm (d) and nucleus (e) in cells treated as described in (c) compared with the cytoplasmic marker β-actin and the nuclear marker HDAC1, respectively. Each value is presented as the mean ± SD from three independent experiments. *P < 0.05, **P < 0.01 compared with control group

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