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Fig. 3 | Respiratory Research

Fig. 3

From: Knockdown of annexin A5 restores gefitinib sensitivity by promoting G2/M cell cycle arrest

Fig. 3

ANXA5 silencing promotes G2/M arrest and apoptosis in gefitinib-resistant cells through caspase activation and mitochondrial damage. a-b PC9R cells expressing ANXA5 shRNA or shNEG were treated with 1 μM gefitinib, stained with FITC-annexin V/propidium iodide, and then analyzed by flow cytometry. ANXA5 silencing increased apoptosis. c-d PC9R cells expressing ANXA5 shRNA or shNEG were treated with 1 μM gefitinib, and cell cycle distribution was assessed by flow cytometry. e-f Representative histograms of JC-1 staining as measured by flow cytometry. ANXA5 silencing increased the proportion of monomeric JC-1 in ANXA5-depleted cells, following incubation with 1 μM gefitinib. g-i Western blot of cleaved caspase-3 and cleaved PARP in PC9R cells expressing shANXA5 or shNEG. Data are representative of three similar experiments. *, P < 0.05 vs. negative control

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