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Fig. 6 | Respiratory Research

Fig. 6

From: The anti-proliferative and anti-inflammatory response of COPD airway smooth muscle cells to hydrogen sulfide

Fig. 6

Effect of mitogen-activated protein kinase 1/2 (MEK-1/2) and p38 MAPK inhibition upon FCS-induced proliferation, IL-6 and CXCL8 release in human ASM cells from non-smokers, smokers and COPD patients. ASM cells were incubated with an MEK-1/2 (5 mM) inhibitor, a p38 (5 mM) inhibitor, or both for 30 min. The ASM cells were then further incubated with NaSH (100 μM) for another 8 days. DNA synthesis (a), IL-6 release (b), and CXCL8 release (c) were subsequently measured by bromodeoxyuridine (BrdU) ELISA, and DuoSet ELISA respectively. Bars represent means (± SEM) of nine ASM donors. *P < 0.05 versus cells plus 2.5% FCS; **P < 0.01 versus cells plus 2.5% FCS; ***P < 0.001 versus cells plus 2.5% FCS; #P < 0.05 versus cells plus 2.5% FCS plus NaSH; ##P < 0.01 versus cells plus 2.5% FCS plus NaSH

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