Fig. 7
From: Resolution of bleomycin-induced murine pulmonary fibrosis via a splenic lymphocyte subpopulation
Adoptively transferred Tregs reduced chemokine (CC motif) ligand-2 (CCL2) production and fibrocyte accumulation. a CCL2 levels in lung digests were measured by performing ELISA on day 28 post-BLM challenge. CCL2 production was significantly increased in BLM-treated mouse lungs compared with that in lungs from saline-treated and Treg-transferred mice. Adoptive transfer of Tregs significantly attenuated CCL2 production in BLM-treated mice. *P < 0.05, ** P < 0.01. n = 3 mice/group. NC; normal control. b–g Immunohistological analyses for collagen-I (Col-I; green; b and e) and CD45 (red; c and f) were performed with lung sections on day 28 after BLM treatment, with or without adoptive Treg transfer. The merged images (yellow; d and g) represent co-staining for Col-I and CD45, indicating the presence of bone marrow-derived fibrocytes. Fibrocytes are indicated with arrows. The increased numbers of fibrocytes expressing Col-I and CD45 observed in BLM-treated mice were attenuated by adoptive Treg transfer. Nuclei were counterstained with 4′,6-diamidino-2-phenylindole. Original magnification, × 40