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Table 1 Demographics of the study population and biologic samplesa

From: Altered lung biology of healthy never smokers following acute inhalation of E-cigarettes

Parameter Total cohort of n = 10 Sub-cohort exposed to EC with nicotine, n = 7 Sub-cohort exposed to EC without nicotine, n = 3
Baselineb Post-ECc Baselineb Post-ECc Baselineb Post-ECc
n 10 7 3
Sex (male/female) 5/5 4/3 1/2
Age (yr) 40.2 ± 9.7 40.4 ± 11.2 39.7 ± 6.7
Race (B/W/H/O)d 7/0/3/0 6/0/1/0 1/0/2/0
Smoking history None None None
Pulmonary functione
 FVC (% predicted) 110 ± 14 107 ± 13 112 ± 16 112 ± 11 105 ± 6 98.3 ± 12
 FEV1 (% predicted) 110 ± 14 107 ± 15 112 ± 15 113 ± 11 103 ± 9 91 ± 8
 FEV1/FVC (% observed) 81 ± 33 81 ± 4 81 ± 3 83 ± 3 81 ± 4 76 ± 4
 TLC (% predicted) 92 ± 11 92 ± 11 91 ± 11 92 ± 7 94 ± 13 91 ± 21
 DLCO (% predicted) 89 ± 10 86 ± 10 88 ± 10 85 ± 13 92 ± 9 87 ± 3
 O2 saturation 99 ± 1 99 ± 1 99 ± 1 99 ± 1 99 ± 2 98 ± 1
Bronchoalveolar lavage
 % recovery 61.7% 64.8% 64.4% 65.5% 56.2% 62.9%
 Total cells recovered (x106)f 12.1 ± 7.9 10.0 ± 6.7 10.6 ± 5.3 11.0 ± 7.6 15.5 ± 13.1 6.6 ± 2.5
 Epithelial cells (%)g 87.0 ± 9.0 1.6 ± 1.7 1.1 ± 1.0 2.0 ± 1.8 0.3 ± 0.3 0.6 ± 0.5
 Macrophages (%)g 92.7 ± 7.4 90.0 ± 5.7 91.1 ± 8.3 90.0 ± 6.2 96.6 ± 2.7 90.1 ± 5.6
 Lymphocytes (%)g 5.8 ± 6.5 7.8 ± 4.8 7.2 ± 7.4 7.5 ± 4.7 2.6 ± 2.1 8.5 ± 6.2
 Neutrophils (%)g 0.5 ± 0.5 0.50 ± 0.3 0.6 ± 0.5 0.5 ± 0.2 0.3 ± 0.4 0.5 ± 0.7
 Eosinophils (%)g 0.1 ± 0.2 0.2 ± 0.2 0.1 ± 0.2 0.1 ± 0.2 0.1 ± 0.2 0.3 ± 0.1
Small airway epitheliumh
 Epithelial cells (%) 99.1 ± 1.0 99.3 ± 1.0 99.2 ± 1.0 99.4 ± 0.5 98.9 ± 1.2 99.0 ± 1.8
 Inflammatory cells (%) 0.86 ± 1.0 0.7 ± 1.0 0.8 ± 1.0 0.6 ± 0.5 1.1 ± 1.2 1.0 ± 1.8
 Ciliated cells (%) 60.1 ± 4.5 58.3 ± 7.5 58.6 ± 4.1 58.3 ± 6.8 63.7 ± 3.4 58.2 ± 10.9
 Secretory cells (%) 10.5 ± 3.4 11.1 ± 2.3 9.2 ± 2.4 10.7 ± 2.0 13.4 ± 4.1 12.1 ± 3.0
 Undifferentiated cells (%) 22.3 ± 4.3 21.9 ± 6.6 22.6 ± 4.7 20.2 ± 4.3 23.3 ± 3.3 26.0 ± 10.1
 Basal cells (%) 6.92 ± 3.7 7.9 ± 5.9 8.7 ± 2.7 10.3 ± 5.5 2.8 ± 2.2 2.5 ± 1.3
  1. aAll subjects were healthy never smokers; values shown are mean ± standard deviation unless otherwise noted
  2. bBaseline – assessment at baseline prior to EC exposure
  3. cPost-EC – assessment 1 week after baseline, following 2 × 10 puffs of Blu brand electronic cigarette with (n = 7 subjects) or without (n = 3 subjects) nicotine
  4. dEthnicity is indicated as: Black (B), White (W), Hispanic (H), Other (O)
  5. eLung function values shown are pre-bronchodilator. FVC - forced vital capacity; FEV1 - forced expiratory volume in 1 s; DLCO - diffusion capacity for carbon monoxide; TLC - total lung capacity; all values are presented as % predicted except for FEV1/FVC presented as % observed
  6. f% recovery – volume recovered / volume infused
  7. gDifferential cell count prior to enrichment of alveolar macrophages (see Methods for details); after enrichment, alveolar macrophages represent ≥98% of the cell population used for RNA sequencing
  8. hCell differentials of the small airway epithelium used for RNA sequencing