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Fig. 1 | Respiratory Research

Fig. 1

From: Human alveolar macrophages predominately express combined classical M1 and M2 surface markers in steady state

Fig. 1

Comparison of human alveolar macrophage phenotype between UK and Malawi healthy adults (a) Percentages of AM subsets gated for CD206 and CD86 (UK cohort, n = 9 for grey bars and n = 16 for black bars) and the (b) the degree of CD163 expression in each of the subsets. The data shows that CD206hiCD86hi subset occupies the highest proportion and expresses the highest levels of CD163 when compared with the other three. c-d The same pattern is observed on the AM derived from the healthy Malawian individuals (grey bars, n = 10). The effect of pneumococcal colonization and HIV infection on AM polarisation. a-b AM subsets are compared between non-colonized (carriage -) and colonized group (carriage +) post experimental nasal pneumococcal inoculation. There is no alteration of AM phenotype associated with nasal pneumococcal colonization. CD206hiCD86hi is the dominant subset with the highest CD163 expression amongst the rest (p < 0.0001 when compared with CD206loCD86hi or CD206hiCD86lo. c AM subsets are compared between HIV-uninfected healthy adults (n = 10) and HIV-infected individuals (n = 10). AM collected from HIV-infected individuals follows the same pattern with healthy HIV-uninfected individuals when gated for CD206 and CD86. d CD206hiCD86hi is the dominant subset with the highest CD163 expression amongst the rest (p < 0.002 when compared with CD206loCD86hi or CD206hiCD86lo in both HIV-uninfected and HIV-infected individuals. However, the HIV infection regresses the expression of CD163 in the CD206loCD86hi subset. e-g Levels of CD80, CD64 and HLADR expression respectively between carriage negative and carriage positive individuals. There is no significant difference on levels of CD80, CD64 and HLADR expression mediated by nasopharynx pneumococcal colonization. Within-group comparisons used Wilcoxon tests, and between-cohort comparisons used Mann-Whitney U test

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