Fig. 4

Total protein nitration in plasma samples of AA-treated rats and from PAH patients. Densitometric analysis indicates that AA injections induced an accumulation of plasma nitrated protein, which become significant by day 12 of study (a). Representative dot for day 24 was taken from different part of the same membrane. The levels of the plasma protein nitration in females but not male patients with PAH positively correlate with pulmonary vascular resistance (PVR, WU, b and negatively – with cardiac output (L/min, c), suggesting the nitrosative stress could be involved in PAH progression in females. a - data are mean ± SEM, N = 3–5 per group. *P < 0.05 vs. Control using unpaired t-test; b, c - N = 12–29 per group, Person coefficient and P-value were calculated by two-tail correlation test