Table 2 Primary antibodies used in Western Blot analysis, Immunofluorescence and Proximity Ligation Assays
From: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis
Target | Abbreviation | Antibody | Provider | Application |
|---|---|---|---|---|
β-actin | ACTB | HRP-conjugated anti-ACTB antibody | Sigma Aldrich, St. Louis, USA | WB |
Calpain-4 | CAPNS1 | mouse monoclonal anti-Calpain-4 | Abnova, Taipei City, Taiwan | WB, IF, PLA |
Caveolin-1 | CAV1 | rabbit monoclonal anti-Caveolin-1 antibody | Cell Signaling, Boston, USA | WB |
Collagen VI α1 | COL6A1 | mouse monoclonal anti-Collagen VI A1 antibody | Santa Cruz, Dallas, USA | WB, IF, PLA |
Collagen VI α3 | COL6A3 | mouse monoclonal anti-Collagen VI A3 antibody | Santa Cruz, Dallas, USA | IF, PLA |
Coronin 1C | CORO1C | mouse monoclonal anti-CORO1C antibody | Santa Cruz, Dallas, USA | WB, IF, PLA |
Extracellular Signaling Related Kinase 1 | ERK1 | mouse monoclonal anti-ERK1 antibody | BD Biosciences, New Jersey, USA | WB |
ER protein 57 (Protein disulfide-isomerase A3) | ERp57 | mouse monoclonal anti-ERp57 | Abcam, Cambridge, UK | PLA |
Fibulin-1 | FBLN1 | mouse monoclonal anti-FBLN1 antibody | Santa Cruz, Dallas, USA | WB, IF, PLA |
FK506-binding protein 10 | FKBP10 | rabbit polyclonal anti-FKBP10 antibody | ATLAS, Stockholm, Sweden | WB, IF, PLA |
Focal Adhesion Kinase | FAK | rabbit polyclonal anti-FAK antibody | Santa Cruz, Dallas, USA | WB |
Golgin97 | CDF4 | mouse monoclonal anti-Golgin97 antibody | Invitrogen, Carlsbad, USA | PLA |
mouse IgG (neg. ctrl) | mouse IgG | mouse IgG1κ isotype control | eBioscience, San Diego, USA | PLA |
Integrin-β1 | ITGB1 | mouse monoclonal anti-ITGB1 antibody | Abcam, Cambridge, UK | WB |
Phosphorylated Extracellular Signaling Related Kinase 1/2 | p-ERK1/2 | rabbit monoclonal anti-pERK1/2 (Thr202/Tyr204) | Cell Signaling, Boston, USA | WB |
Phosphorylated Focal Adhesion Kinase | p-FAK Y397 | rabbit monoclonal anti-pFAK (Tyr397) | Cell Signaling, Boston, USA | WB |
Phosphorylated Focal Adhesion Kinase | p-FAK Y566/577 | rabbit monoclonal anti-pFAK (Tyr576/Tyr577) | Biomol, Hamburg, Germany | WB |
Phosphorylated SRC Proto-Oncogene, Non-Receptor Tyrosine Kinase | p-Src | rabbit polyclonal anti-pSrc (Tyr416) | Cell Signaling, Boston, USA | WB |
SRC Proto-Oncogene, Non-Receptor Tyrosine Kinase | Src | mouse monoclonal anti-Src | Cell Signaling, Boston, USA | WB |
Talin 1 | TLN1 | mouse monoclonal anti-TLN1 | Sigma Aldrich, Dt. Louis, USA | WB, IF, PLA |