Skip to main content

Advertisement

Table 2 Primary antibodies used in Western Blot analysis, Immunofluorescence and Proximity Ligation Assays

From: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

Target Abbreviation Antibody Provider Application
β-actin ACTB HRP-conjugated anti-ACTB antibody Sigma Aldrich, St. Louis, USA WB
Calpain-4 CAPNS1 mouse monoclonal anti-Calpain-4 Abnova, Taipei City, Taiwan WB, IF, PLA
Caveolin-1 CAV1 rabbit monoclonal anti-Caveolin-1 antibody Cell Signaling, Boston, USA WB
Collagen VI α1 COL6A1 mouse monoclonal anti-Collagen VI A1 antibody Santa Cruz, Dallas, USA WB, IF, PLA
Collagen VI α3 COL6A3 mouse monoclonal anti-Collagen VI A3 antibody Santa Cruz, Dallas, USA IF, PLA
Coronin 1C CORO1C mouse monoclonal anti-CORO1C antibody Santa Cruz, Dallas, USA WB, IF, PLA
Extracellular Signaling Related Kinase 1 ERK1 mouse monoclonal anti-ERK1 antibody BD Biosciences, New Jersey, USA WB
ER protein 57 (Protein disulfide-isomerase A3) ERp57 mouse monoclonal anti-ERp57 Abcam, Cambridge, UK PLA
Fibulin-1 FBLN1 mouse monoclonal anti-FBLN1 antibody Santa Cruz, Dallas, USA WB, IF, PLA
FK506-binding protein 10 FKBP10 rabbit polyclonal anti-FKBP10 antibody ATLAS, Stockholm, Sweden WB, IF, PLA
Focal Adhesion Kinase FAK rabbit polyclonal anti-FAK antibody Santa Cruz, Dallas, USA WB
Golgin97 CDF4 mouse monoclonal anti-Golgin97 antibody Invitrogen, Carlsbad, USA PLA
mouse IgG (neg. ctrl) mouse IgG mouse IgG1κ isotype control eBioscience, San Diego, USA PLA
Integrin-β1 ITGB1 mouse monoclonal anti-ITGB1 antibody Abcam, Cambridge, UK WB
Phosphorylated Extracellular Signaling Related Kinase 1/2 p-ERK1/2 rabbit monoclonal anti-pERK1/2 (Thr202/Tyr204) Cell Signaling, Boston, USA WB
Phosphorylated Focal Adhesion Kinase p-FAK Y397 rabbit monoclonal anti-pFAK (Tyr397) Cell Signaling, Boston, USA WB
Phosphorylated Focal Adhesion Kinase p-FAK Y566/577 rabbit monoclonal anti-pFAK (Tyr576/Tyr577) Biomol, Hamburg, Germany WB
Phosphorylated SRC Proto-Oncogene, Non-Receptor Tyrosine Kinase p-Src rabbit polyclonal anti-pSrc (Tyr416) Cell Signaling, Boston, USA WB
SRC Proto-Oncogene, Non-Receptor Tyrosine Kinase Src mouse monoclonal anti-Src Cell Signaling, Boston, USA WB
Talin 1 TLN1 mouse monoclonal anti-TLN1 Sigma Aldrich, Dt. Louis, USA WB, IF, PLA
  1. Secondary HRP-linked antibodies and secondary antibodies (Alexa Fluor 488 goat anti-mouse IgG, Alexa Fluor 568 goat anti-rabbit IgG) for IF were purchased from GE Healthcare Life Sciences (Freiburg, Germany). 4',6-Diamidino-2-phenylindole (DAPI) was used for nuclear staining (Sigma-Aldrich, St. Louis, USA)