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Fig. 8 | Respiratory Research

Fig. 8

From: Acute lung injury by gastric fluid instillation: activation of myofibroblast apoptosis during injury resolution

Fig. 8

Lung tissue TIMP-2 expression by Western blot after a single instillation of gastric fluid. a Representative Western blot of lung tissue homogenate illustrating the time course of changes in TIMP-2 expression. This immunoblot is from a single set of animals (one animal sample per time point) and is representative of 5 separate sets. The approximately 30 kDa band density corresponding to TIMP-2 seen in the control sample increases significantly at day 4 and is similar to the control band at days 7 and 14. β-tubulin immunoblot shows equal protein loading. b Densitometric analysis of five independent Western blots showing a significant progressive reduction in TIMP-2 after a significant increment at day 4. Western blot bands were normalized to beta-tubulin. Data are means ± SD. *: p < 0.05 with respect to controls. rS= Spearman rank correlation coefficient for the reduction in band size from days 4 to 14

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