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Fig. 4 | Respiratory Research

Fig. 4

From: Acute lung injury by gastric fluid instillation: activation of myofibroblast apoptosis during injury resolution

Fig. 4

Cleaved caspase-3 location in intra-alveolar buds of granulation tissue after a single gastric fluid instillation. Representative images of indirect immunofluorescence of normal lung and of lung studied at days 4, 7 and 14 after a single orotracheal instillation of gastric fluid using antibodies to cleaved caspase-3 (green) and α-SMA (red) as primary antibodies. Cell nuclei are co-stained (blue) with DAPI (4′,6-diamidino-2-phenylindole). Evidence of caspase-3 activation is present in intra-alveolar bud-α-SMA –positive and –negative cells at days 4 and 7 only. Arrows indicate α-SMA-positive cells that are also positive for cleaved caspase-3. Arrowheads indicate α-SMA-negative cells that are positive for cleaved caspase-3. Original magnification 630X

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