Table 1 Sequences of primers used for RT-qPCR to assess expression of the indicated genes
Gene | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|
Cyclophilin A | TTCCTCCTTTCACAGAATTATTCCA | CCGCCAGTGCCATTATGG |
Beta-2-microglobulin | CTTTCTGGTGCTTGTCTCACTGA | GTATGTTCGGCTTCCCATTCTC |
GAPDH | CAACTCACTCAAGATTGTCAGCAA | TGGCAGTGATGGCATGGA |
RPLP0 | GGACCCGAGAAGACCTCCTT | GCACATCACTCAGAATTTCAATGG |
GUSB | CATTAGCAAGCTGGTCCAGAGT | GACAAAGTAACCCTTGGGATACAT |
MuRF1 | CTTCCTCTCAAGTGCCAAGCA | GTGTTCTAAGTCCAGAGTAAAGTAGTCCAT |
Atrogin-1 | CAGCAGCTGAATAGCATCCAGAT | TCTGCATGATGTTCAGTTGTAAGC |
LC3B | GAGCAGCACCCCACCAAGAT | CGTGGTCAGGCACCAGGAA |
p62/SQSTM1 | GAATGTGGGGGAGAGTGTGG | TCTTCTGTGCCTGTGCTGGA |
REDD1 | TCGGCGCTTCACTACTGACC | CCTAACACCCACCCCATTCC |
FoXO1 | AAGAGCGTGCCCTACTTCAAGGATA | CCATGGACGCAGCTCTTCTC |
IL-6 | GTATGAACAACGATGATGCACTTG | GAAGACCAGAGGAAATTTTCAATAGG |
TNF-α | CAGCGCTGAGGTCAATCTGCC | TGCCCGGACTCCGCAA |
CXCL1 | TCGTCTTTCATATTGTATGGTCAACACG | TGCCCTACCAACTAGACACAAAATGTC |