Fig. 5

Dynamic regulation of FoXO1 activity during muscle atrophy and recovery following pulmonary inflammation. UBWT and K48R mice were subjected to the intratracheal instillation of NaCl or LPS, and gastrocnemius muscle was collected at the indicated time points (hours after instillation). Protein levels of phosphorylated Akt (ser473), total Akt, phosphorylated FoXO1 (ser256) and total FoXO1 were assessed in lysates of gastrocnemius muscle tissue with western blot analysis. b, d) Representative western blots of the indicated proteins. Ratios of (a) phosphorylated Akt over total Akt and (c) phosphorylated FoXO1 over total FoXO1. e) mRNA transcript levels of FoXO1 were determined, normalized to geNorm, and expressed as fold change compared with UBWT intratracheal NaCl. * p < 0.05, ** p < 0.01 compared with control (intratracheal NaCl), * above a line refers to a difference in response between genotypes