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Fig. 4 | Respiratory Research

Fig. 4

From: Pulmonary inflammation-induced loss and subsequent recovery of skeletal muscle mass require functional poly-ubiquitin conjugation

Fig. 4

Increased REDD1 expression accompanies inhibited protein synthesis signaling following pulmonary inflammation. UBWT and K48R mice were subjected to the intratracheal instillation of NaCl or LPS, and gastrocnemius muscle was collected at the indicated time points (hours after instillation). Protein levels of phosphorylated mTOR (ser2448), total mTOR or (c) REDD1 were assessed in lysates of gastrocnemius muscle tissue with western blot analysis. a) Ratio of phosphorylated mTOR over total mTOR. b) Representative western blots of the indicated proteins. d) mRNA transcript levels of REDD1 were determined, normalized to geNorm, and expressed as fold change compared with UBWT intratracheal NaCl. *** p < 0.001 compared with control (intratracheal NaCl)

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