Fig. 1

Pulmonary inflammation-induced muscle atrophy and subsequent muscle mass recovery require poly-ubiquitin conjugation. UBWT and K48R mice were instilled intratracheally with NaCl or LPS. At 48 h after LPS a) lung sections were stained with H&E to confirm pulmonary inflammation, or (B) mRNA levels of inflammatory markers IL-6, TNFα and CXCL1 were determined in lung tissue from WT and K48R mice. At 7 (UBWT only), 24 and 120 h after LPS, mRNA abundance of (c) MuRF1 and (d) Atrogin-1 were assessed in gastrocnemius muscle tissue. e) Gastrocnemius wet weights were measured and expressed as a percentage of their respective IT-NaCl time control to represent the response to pulmonary inflammation. All data shown represent means ±SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with control (intratracheal NaCl), * above a line refers to a difference in response between genotypes. # represents a trend