Fig. 1

Effect of H₂O₂ on the expression of Interleukin-33 (IL-33) in NCI-H292 cells. a-c Effect of H₂O₂ on the gene expression of IL-33 and the cell viability in NCI-H292 cells. a, b Cells were treated with various concentration of H₂O₂. After 4 h incubation, whole cells were harvested and assayed for the gene expression of IL-33 (a) and cell viability (b). c Cells were treated with 200 μM H₂O₂. At various time points after the incubation, whole cells were harvested and assayed. d, e Evaluation of nuclear fraction of IL-33 protein. At various time points after the treatment with 200 μM H₂O₂, the nuclear fraction of cell lysates was obtained. d IL-33 protein in the nuclear fraction was evaluated by immunoblotting. e Each band intensity was assessed by densitometry. Relative intensity was calculated by dividing the IL-33 band intensity by each appropriate lamin A/C band intensity. f Effect of N-acethylcysteine (NAC) on H₂O₂-augmented IL-33 expression. Values are the mean ± SEM (n = 3 - 5). *p < 0.05, ***p < 0.001 compared with the values of vehicle-treated cells