Fig. 4
From: Modulation of human airway smooth muscle biology by human adipocytes
Cytokine synthesis by ASMCs in presence of adipocytes. a Eotaxin and IL-6 production by ASMCs when stimulated with serum-free, non-conditioned media (CTRL), intrathoracic (i) and extrathoracic (e) adipocyte conditioned media for 24 h. b represents Eotaxin and IL-6 released by ASMCs when cultured in cell-free 10% FBS supplemented RPMI media (CTRL), intrathoracic (i) and extrathoracic (e) adipocytes for 3 days. Data represented as mean ± SEM (n = 6 donors), repeated measures one-way ANOVA. *indicates significant difference from CTRL, P < 0.05. IL-13 and cytomix were used as positive controls for assay validation that allowed significant release of IL-6 (31.98 ± 9.2 ng/ml) and eotaxin (74 ± 19 pg/mL) in by ASMCs respectively (data not plotted)