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Fig. 4 | Respiratory Research

Fig. 4

From: Endothelial Cdc42 deficiency impairs endothelial regeneration and vascular repair after inflammatory vascular injury

Fig. 4

Cdc42 regulates cell proliferation and migration and angiogenesis during the repair process. a CCK8 assay showing that cell viability recovery peaked at 48 h after LPS-induced injury in control cells. Representative OD values are shown. In addition, siCdc42 and InhCdc42 cells displayed decreased viability compared to control cells at the same time point. n = 4. P < 0.05 (Student’s t test). b Representative micrographs demonstrating the proliferative capacity of ECs in the control, siCdc42 and InhCdc42 groups at 48 h after LPS challenge. The cells were stained with Alexa540-conjugated anti-BrdU antibodies and appear red. The nuclei were stained with DAPI and appear blue, n = 4. Scale bar, 100 μm. c Graphic presentation of the significant differences in cell proliferation between the groups, n = 4. *P < 0.05 **P < 0.01 (Student’s t test). d Representative micrographs of cell migration at 48 h after LPS challenge, n = 4. Scale bar, 250 μm. e Graphic presentation of the portion of the scratch line across which the cells had migrated, n = 4. **P < 0.01 (Student’s t test). f Representative micrographs showing vascular repair at baseline (48 h after seeding in the Matrigel-coated wells) and 48 h after LPS challenge. The live cells were stained with CFSE to enhance their visibility (green), n = 4. Scale bar, 250 μm (g, h) Graphic presentation of the numbers of tubes and networks per field, n = 4. **P < 0.01 (Student’s t test)

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