Fig. 5

Bleomycin induced lung fibrosis and JAK2 and STAT3 activation. Wistar rats were administered a single intratracheal dose of bleomycin (BLM; 3.75 U/kg) on day 1. JSI-124 (1 mg/kg/day) or vehicle was administered intraperitoneally from day 14 until the analysis at day 28 (n = 10 per group). Lung tissue from the vehicle control, BLM, and BLM + JSI-124 groups was immunostained for JAK2, p-JAK2, STAT3, and p-STAT3 (brown) and counterstained with hematoxylin. Representative (a) immunohistochemistry and (b) western blot images are shown. Scale bar = 100 μm. The IgG isotype control was negative (data not shown). Data are expressed as the ratio to β-actin for protein levels. The results are expressed as the mean (SEM), n = 10. One-way ANOVA followed by post-hoc Bonferroni tests. *P < 0.05 vs. controls; ^#P < 0.05 vs. BLM