Fig. 4

Effect of OSA-like IH on the inflammatory response in melanoma lung metastasis mice. qRT-PCR analysis of TNF-α (A) and IL-6 (B) mRNA expression (n = 6 per group) and western blotting analysis of NF-κB P65 protein expression (C a) in tumor tissue from mouse lungs after OSA-like IH exposure and/or tempol treatment (n = 3 per group). The expression level of NF-κB P65 (C b) was evaluated by densitometric analysis of western blot bands. All data are expressed as the mean ± SEM. *P < 0.05