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Fig. 6 | Respiratory Research

Fig. 6

From: Role and regulation of Abelson tyrosine kinase in Crk-associated substrate/profilin-1 interaction and airway smooth muscle contraction

Fig. 6

CAS/Pfn-1 coupling and cortactin/Pfn-1 interaction are regulated by phosphorylation. a HASM cells expressing wild type (WT) CAS and CAS-SD mutant were stimulated with ACh (100 μM, 5 min), or left unstimulated. The protein-protein interaction was evaluated by co-immunoprecipitation. Data are mean ± SE (n = 4). b ACh-induced interaction of cortactin with Pfn-1 is enhanced in cells expressing non-phosphorylated CAS mutant. Cortactin/Pfn-1 interaction was evaluated for HASM cells expressing CAS-WT and CAS-SD. Data are mean ± SE (n = 4). c Association of CAS with Pfn-1 upon ACh stimulation is increased in cells expressing non-phosphorylated cortactin mutant. CAS/Pfn-1 coupling was assessed for HASM cells expressing cortactin-NP. Values are mean ± SE (n = 4). NP, non-phosphorylated; cort, cortactin. **P < 0.01

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Respiratory Research

ISSN: 1465-993X

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