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Fig. 5 | Respiratory Research

Fig. 5

From: Role and regulation of Abelson tyrosine kinase in Crk-associated substrate/profilin-1 interaction and airway smooth muscle contraction

Fig. 5

Effects of CAS-peptide on CAS phosphorylation, F/G-actin ratios, contraction and myosin phosphorylation. a Mouse tracheal rings pretreated with control or CAS peptide were stimulated with ACh (100 μM, 5 min), or left unstimulated. CAS phosphorylation at Tyr-410 was determined by immunoblot analysis. Data are mean ± SE (n = 4). b Mouse tracheal tissues that had been pretreated with control or CAS peptide were stimulated with ACh or left untreated. F/G-actin ratios in tissues were evaluated using the fractionation assay. Values represent mean ± SE (n = 5). c Contractile response of mouse tracheal rings to ACh (10−5 M) was determined, after which they were treated with peptides (2.5 μg/ml) for 30 min. They were then stimulated with different concentration of ACh. Contractile force is normalized to contraction induced by 10−5 M ACh before treatment with the peptides (n = 7–8). d Myosin light chain phosphorylation in mouse tracheal segments pretreated with peptides was assessed by immunoblot analysis. Values represent mean ± SE (n = 4). **P < 0.01; NS, not significant

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