Fig. 2

c-Abl knockout inhibits actin filament polymerization in response to ACh stimulation. a Mouse tracheal rings from c-Abl^-lox and c-Abl^smko mice were treated with acetylcholine (ACh) (100 μM, 5 min) or left untreated (UT). F/G-actin ratios were evaluated using the fractionation assay. Data are mean ± SE (n = 5). b Representative images illustrating the effects of c-Abl knockout on F/G-actin ratios. Sections of trachealis from c-Abl^-lox and c-Abl^smko mice were stained with DNase I (for G-actin) or phalloidin (for F-actin). c ACh-induced increases in F/G-actin ratios evaluated by fluorescent microscopy are reduced in c-Abl^smko mice (n = 4). *P < 0.05, **P < 0.01