Fig. 1
Increases in the association and translocation of CAS with Pfn-1 upon acetylcholine stimulation are regulated by c-Abl. a Mouse tracheal rings from c-Abl-lox and c-Ablsmko mice were treated with acetylcholine (ACh) (100 μM, 5 min) or left untreated (UT) followed by CAS immunoprecipitation. CAS immunoprecipitates were separated by SDS-PAGE and blotted with antibodies against CAS and Pfn-1. Data are mean ± SE (n = 4). b Human airway smooth muscle (HASM) cells expressing control shRNA or c-Abl shRNA were stimulated with ACh (100 μM, 5 min) or left untreated. The cellular localization of CAS and Pfn-1 was evaluated by immunofluorescent microscopy. Arrows indicate a single line scan to analyze the fluorescent intensity for each cell. The inset plots represent fluorescent intensity of the line scan indicated by the arrows. c Fluorescent ratios of cell periphery over interior for CAS or Pfn-1 in cells were calculated. Data are mean ± SE (n = 26–27 cells from 4 independent experiments). UT, untreated. ** P < 0.01