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Fig. 5 | Respiratory Research

Fig. 5

From: GM-CSF overexpression after influenza a virus infection prevents mortality and moderates M1-like airway monocyte/macrophage polarization

Fig. 5

Characterization of the changes in transcriptome patterns of airway macrophages during IAV infection. BAL airway macrophages were sorted using the gating strategy described in Fig. 4a and next generation RNA-sequencing was used to profile the complete transcriptome data of AMs (a, orange bars) and EMs (b, blue bars) at 8 dpi, the time point at which the survival curves diverge (n = 5 mice per group). The effect of supraphysiologic GM-CSF levels on each of the 43,628 sequenced macrophage genes was examined: differential gene expression was determined using with transcripts having a q-value <0.2 being included. The relative expression of each transcript was calculated using the equation, Log2 Expression Ratio (DTGM:LM) = Log2 \( \Big(\overline{X} \) transcriptDTGM) - Log2 (\( \overline{X} \) transcriptLM), and the differential expression of transcripts is shown. To investigate the impact of GM-CSF on M1/M2 macrophage polarization, the Log2 Expression Ratios were plotted against known M1 and M2 macrophage-associated transcripts from AMs (c, d) and EMs (e, f)

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