Fig. 5From: GM-CSF overexpression after influenza a virus infection prevents mortality and moderates M1-like airway monocyte/macrophage polarizationCharacterization of the changes in transcriptome patterns of airway macrophages during IAV infection. BAL airway macrophages were sorted using the gating strategy described in Fig. 4a and next generation RNA-sequencing was used to profile the complete transcriptome data of AMs (a, orange bars) and EMs (b, blue bars) at 8 dpi, the time point at which the survival curves diverge (n = 5 mice per group). The effect of supraphysiologic GM-CSF levels on each of the 43,628 sequenced macrophage genes was examined: differential gene expression was determined using with transcripts having a q-value <0.2 being included. The relative expression of each transcript was calculated using the equation, Log2 Expression Ratio (DTGM:LM) = Log2 \( \Big(\overline{X} \) transcriptDTGM) - Log2 (\( \overline{X} \) transcriptLM), and the differential expression of transcripts is shown. To investigate the impact of GM-CSF on M1/M2 macrophage polarization, the Log2 Expression Ratios were plotted against known M1 and M2 macrophage-associated transcripts from AMs (c, d) and EMs (e, f)Back to article page