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Fig. 4 | Respiratory Research

Fig. 4

From: GM-CSF overexpression after influenza a virus infection prevents mortality and moderates M1-like airway monocyte/macrophage polarization

Fig. 4

Flow cytometric discrimination of alveolar and exudative macrophages by surface marker expression. Representative FACS plots from an IAV-infected LM mouse at 10 dpi, which detail our 12-color flow cytometry gating strategy of single cell suspensions from BAL and enzyme-digested lung (a). Alveolar macrophages (AM) were designated as F4/80+ SiglecF+ CD11bneg/dim, whereas exudative macrophages (EM) were designated as F4/80+ SiglecFneg/dim CD11b+. Supra-physiologic GM-CSF levels during IAV infection had no effect on the absolute number of either airway (BAL-recovered) AM or EM cell numbers at 10 dpi (b)

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