Fig. 3From: GM-CSF overexpression after influenza a virus infection prevents mortality and moderates M1-like airway monocyte/macrophage polarizationEffects of supra-physiologic levels of GM-CSF on bronchoalveolar lavage fluid content and influenza A virus load. BAL fluid was recovered from mice at indicated time points after IAV infection and total protein (a) was measured by BCA assay, while BAL concentrations of alpha-2-macroglobulin (b) and amphiregulin (c) levels were quantitated by ELISA. The number of influenza A virus matrix protein (M1) transcripts was quantitated from whole lung by RT-PCR (d). Results shown represent three independent experiments (*p < 0.05, ***p < 0.0005)Back to article page