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Fig. 9 | Respiratory Research

Fig. 9

From: Lack of SOCS3 increases LPS-induced murine acute lung injury through modulation of Ly6C(+) macrophages

Fig. 9

Depletion of circulating Ly6C(+) monocytes suppressed acute lung injury and inflammation in SOCS3(Lyz2cre) (KO) mice after i.t. LPS treatment. 25 μg rat monoclonal anti-mouse Ly6C antibody (Clone: 6C3) and 5 mg/kg LPS were co-administered into mice via i.p. and i.t. respectively (Group anti-Ly6C/LPS, n = 5). The mice received the same doses of IgG and LPS were used as controls (group IgG/LPS, n = 5). 2 days after the treatment, BAL, blood and lung tissues were collected for flow cytometry analysis. a Lung histology analysis by H&E staining. Anti-Ly6C antibody decreased acute lung injury and inflammation. One representative photograph was shown for each group of mice (Upper panel) and quantitatively analysis of the severity of acute lung injury (Lower panel). *p < 0.05 v.s. the IgG control group, n = 5. b Anti-Ly6C antibody moderately suppressed CD11b(+)Ly6C(+) monocytes and CD11b(−)Thy1.2+ T lymphocytes in blood circulation. c Anti-Ly6C antibody moderately suppressed Ly6C(+) macrophages and Ly6G(+) neutrophils in BAL. One representative data of each group was shown. d Quantitative analysis of Ly6G(+) neutrophils, Ly6C(+) macrophages and CCR2(+) expression in lung macrophages by flow cytometry. *p < 0.05 v.s. the IgG control group, n = 5. e CXCL15 protein expression in lung lysates was analyzed by ELISA assay. Data is presented as mean ± standard error

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