Fig. 6

Lack of SOCS3 resulted in lower CD206(+) alternatively activated macrophages (M2 macrophages) and more expression of STAT3 in the lung of mice. a CD206+ Macrophages were gated on F4/80(+)Ly6G(−) cells by flow cytometry. One representative dot plot data shown lower CD206+ M2 macrophages in lung digests of KO mice than the WT mice. b Quantitatively analysis of M2 macrophages. #p < 0.05 v.s. WT/PBS; * p < 0.05 v.s. WT/LPS group, n = 5. c One representative Western blots showed protein levels of total STAT3 and phosphorylated STAT3 at residue Tyr705 in the lung protein extracts. Beta-actin protein was detected as an internal loading control. d The protein expression levels were quantitatively analyzed by Image J software and the data was presented as ratio of target protein densitometric density to beta-actin densitometric density. #p < 0.05 v.s. WT/PBS; * p < 0.05 v.s. WT/LPS group, @p < 0.05 v.s. KO/PBS, n = 5