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Fig. 5 | Respiratory Research

Fig. 5

From: Characterization of pediatric cystic fibrosis airway epithelial cell cultures at the air-liquid interface obtained by non-invasive nasal cytology brush sampling

Fig. 5

CFTR and baseline IL-6 and IL-8 levels in CF and healthy fully differentiated ALI cultures. CFTR mRNA levels (a) assessed by RT-PCR in fully differentiated CF and control ALI cultures. Baseline protein levels of IL-6 (b) and IL-8 (c) in the basolateral chamber of fully differentiated ALI cultures. Data are presented as mean of 5–6 patients/donors. CFTR staining in nasal CF (d, g) nasal healthy (e, h), and bronchial healthy (f, i) cultures at week 4 post exposure to air is shown by representative three-dimensional immunofluorescence staining taken by LSM. xy-projection is shown on top panel and xz-projection is shown on lower panel. Green: CFTR; Red: actin; blue: DAPI (nucleus). The micrographs presented are representative of data generated from 2 to 3 patients/donors

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Respiratory Research

ISSN: 1465-993X

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