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Fig. 3 | Respiratory Research

Fig. 3

From: Characterization of pediatric cystic fibrosis airway epithelial cell cultures at the air-liquid interface obtained by non-invasive nasal cytology brush sampling

Fig. 3

Electron microscopic characterization of differentiated cystic fibrosis cell cultures grown at the air-liquid interface. a-d Ciliated (c), mucus-secreting (m) and basal cells (b) revealed by electron micrographs of a primary culture of cystic fibrosis nasal epithelial cells grown at the air-liquid interface for 4 week. e Electron micrograph of tight junctions (tj) between a ciliated (c) and mucus-secreting (m) cell of a primary culture of cystic fibrosis nasal epithelial cells grown at the air-liquid interface for 4 weeks. f Electron micrograph of a primary culture of cystic fibrosis nasal epithelial cells grown at the air-liquid interface for 4 weeks showing the normal structure of cilia consisting of nine outer 18 microtubule doublets with dynein arms, surrounding one central pair of single microtubules. The micrographs presented are representative from cultures generated from two CF patients

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