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Fig. 2 | Respiratory Research

Fig. 2

From: Characterization of pediatric cystic fibrosis airway epithelial cell cultures at the air-liquid interface obtained by non-invasive nasal cytology brush sampling

Fig. 2

Differentiation of primary cystic fibrosis nasal epithelial cells into a mucociliary phenotype visualized by LSM. a, b Presence of β-tubulin-positive ciliated cells and MUC5AC-positive mucus-secreting cells at 4 weeks post exposure to air was shown by representative three-dimensional immunofluorescence stainings. xy-projection is shown on top panels and xz-projection is shown on lower panels. Blue: DAPI (nucleus); red: actin; green: β-tubulin; white: MUC5AC. c Presence of p63-positive basal cells at week 4 post exposure to air was shown by representative three-dimensional immunofluorescence stainings taken by LSM. xy-projection is shown on top panel and xz-projection is shown on lower panel. Blue: DAPI (nucleus); red: actin; green: β-tubulin; white: p63. d Immunofluorescence staining of the tight junctional protein ZO-1 at 4 weeks post exposure to ALI in the xy-projection. Red: actin; white: ZO-1. The micrographs presented are representative of data generated from six different CF patients

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