Fig. 3
From: A role for B cells in organic dust induced lung inflammation
Impact of ODE-induced cellular influx in lavage fluid and lung tissue compartments in BCR KO animals. WT and BCR KO mice were repetitively exposed to saline or ODE daily for 3 weeks whereupon panel a BALF and panel b dissociated lung tissue cells were processed and analyzed by flow cytometry. BALF total cells were determined by hemocytometer. Total lung cells represent ½ whole lung tissue cells following density gradient separation as determined by hemocytometer. Numbers of neutrophils (Ly6G+), alveolar macrophages (CD11chiCD11blo), exudative macrophages (CD11chiCD11bhi), CD3+CD4+ T cells, and CD3+CD8+ T cells were calculated by multiplying the percentage of cells in respective gate (% of CD45+ cells, as analyzed by FACS) multiplied by respective total cells for each mouse. Bar graphs depict means with standard error bars. N = 6-8 mice/group from 2 independent studies. Statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001) versus saline. Line denotes significant difference (#p < 0.01, ##p < 0.01) between WT and KO