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Fig. 4 | Respiratory Research

Fig. 4

From: Urban particulate matter (PM) suppresses airway antibacterial defence

Fig. 4

PM exposure induced cell senescence via oxidative stress damage. Senescence in BEAS-2B cells after PM exposure was detected by SA-β-gal staining. BEAS-2B cells cultured in a 6-well plate were incubated in medium containing various concentrations of PM as follows: 0, 10, 50, 100 μg/ml, and 50 μg/ml with 5 mM NAC. a, b, c, d and e Representative images (×200) of senescent cells assayed by SA-β-gal staining. f Quantitative analysis of senescent cells. The data are expressed as the mean ± SD of three independent experiments. NAC, N-acetylcysteine; PM, PM-exposed cells. *P < 0.05, **P < 0.01, and ***P < 0.001 versus P0 (0 μg/ml). #P < 0.05 versus P50 (50 μg/ml)

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