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Fig. 3 | Respiratory Research

Fig. 3

From: Urban particulate matter (PM) suppresses airway antibacterial defence

Fig. 3

Effects of PM on intracellular ROS generation upon P. aeruginosa infection. a PM uptake was quantified according to the parameter of side scatter (SSC) intensity in BEAS-2B cells. b and c Intracellular ROS formation is expressed as the DCF mean fluorescence intensity (MFI), and the effect of NAC (d and e) was assessed in GFP-P. aeruginosa-infected BEAS-2B cells. PM significantly dose dependently increased the intracellular ROS generation upon PAO1-infection (b, c, d and e) in BEAS-2B cells. The results are expressed as the mean ± SD of three independent experiments. NAC, N-acetylcysteine; PM, PM-exposed cells; ROS, reactive oxygen species; *P < 0.05 and **P < 0.01 versus P0 (0 μg/ml)

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